How much earlier do Signal Chemistry signals appear vs. TaqMan?

In head-to-head comparisons, Signal Chemistry produces detectable signals 6–8 cycles earlier than TaqMan, with nearly double the endpoint fluorescence on the same hardware.

How many targets can Signal Chemistry detect in one tube?

Up to 30 targets in a single tube has been demonstrated in dPCR. 5-plex / 5-color is demonstrated on standard qPCR; 15-plex on dPCR with just FAM and HEX.

Does Signal Chemistry require new instruments?

No. Signal Chemistry is drop-in compatible with qPCR and dPCR platforms already on your bench. There is no capital purchase, no retraining, and no procurement cycle. It works with FAM and HEX with no new consumables.

How is amplitude-based discrimination tunable?

Because signal generation is decoupled from amplification, each target’s fluorescence intensity can be independently tuned without affecting PCR efficiency. This produces tighter amplitude clusters in 2D dPCR readout plots and supports automated analysis without proprietary cloud software.

How does cost scale with multiplex level?

All assay-specific material is unlabeled. Inexpensive universal probes replace target-specific labeled probes, so per-target cost stays flat as panel size grows — making 30-plex panels economically viable at TaqMan-comparable pricing.

The science behind Signal Chemistry.

Q: What is Signal Chemistry?

Signal Chemistry decouples amplification from signal generation. Standard PCR primers amplify targets while a universal processor drives signal in a secondary isothermal reaction running in parallel. Targets are distinguished by amplitude using just two fluorophores (FAM and HEX) and read on any standard qPCR or dPCR instrument.

Signal Chemistry separates amplification from signal generation — enabling broad multiplex panels on the instruments already on your bench, without adding hardware or complexity.

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Signal Chemistry vs. TaqMan · Amplification Curves · Head-to-Head

What is Signal Chemistry?

Traditional PCR couples amplification and detection into one reaction. Signal Chemistry decouples them — creating a fundamentally different architecture for multiplexing.

Target Amplification

Standard PCR primers amplify targets. No changes to your upstream workflow.

Signal Processing

A signal processor runs in a secondary reaction inside the PCR reaction, in parallel to amplification.

Fluorescent Readout

Targets distinguished by amplitude using FAM and HEX. Read on any standard instrument.

Signal Amplification

Signal Chemistry generates stronger, earlier signals compared to conventional TaqMan — with tunable intensity and maintained linearity.

~2×

Endpoint Fluorescence

Nearly double vs. TaqMan on existing hardware

6–8

Fewer Cycles

Earlier Cq than TaqMan in head-to-head comparison

0.9859

R² Linearity

Superior dilution linearity and predictive power

Signal Chemistry vs. TaqMan · Amplification Comparison Head-to-Head

Signal Chemistry vs TaqMan amplification curves — head-to-head data

Multiplexing Architecture

Amplitude modulation combined with color mixing and universal reagents enables multiplex levels conventional fluorogenic chemistries cannot reach.

How It Scales

Because signal generation is decoupled from amplification, each target's fluorescence level can be independently tuned without affecting PCR efficiency.

5-plex, 5-color demonstrated on standard qPCR platforms
15-plex demonstrated on dPCR with just FAM and HEX
Up to 30 targets in a single tube demonstrated in dPCR
High concordance maintained between singleplex and multiplex reactions
No vendor-specific software or cloud analysis required

Singleplex vs. Multiplex Concordance

Quantitative agreement between singleplex and 4-plex reactions (copies/μL)

Signaling System	Singleplex	Multiplex
FAM Low	178.9	184.8
FAM High	448.9	437.6
HEX Low	127.6	131.5
HEX High	437.6	435.3

15-Plex dPCR · 2 Fluorophores (FAM & HEX) · Single Tube Validated

15-plex dPCR 2D scatter plot showing amplitude-based target discrimination

Platform Advantages

Universal Fluorescent Probe System

A shared pool of fluorogenic probes works across all assays. Adding new targets means adding unlabeled primers — not redesigning the reporter system. Scales to high multiplex without scaling cost.

Earlier, Stronger Signals

Signal appears 6–8 cycles earlier than TaqMan, with nearly double the endpoint fluorescence. Reduces no-template control impact and cuts rain in dPCR — critical for low-copy detection.

Controlled, Tunable Signaling

Signal intensity per target is independently tunable, enabling tighter amplitude clusters in 2D dPCR readout plots. Supports automated analysis without proprietary cloud software.

Flexible Platform Chemistry

Supports both primer-based and probe-based signaling. Use iProbes for hotspot mutation detection, or signal primers for straightforward assay conversion from existing workflows.

Drop-In Compatibility

Works on qPCR and dPCR instruments already on your bench. No new hardware purchases, no retraining, no procurement cycles. Deploy new assays on your existing installed base.

Cost Efficiency at Scale

Inexpensive universal probes replace target-specific labeled probes. Per-target costs stay flat as panel size grows — making 30-plex panels economically viable at TaqMan-comparable pricing.

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